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KMID : 0380020030180040289
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 4 p.289 ~ p.293
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Development of Doxorubicin overproducing Streptomyces Strain using Protoplast Regeneration
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Park Hee-Sub
Park Hyun-Ju
Kim Yong-Hoon
Lim Sang-Min
Kim Dong-Il
Ryu Uk-Sang
Kim Sang-Lin
Kim Eung-Su
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Abstract
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Doxorubicin is an anthracycline-family polyketide compound with a very potent anti-cancer activity, typically produced by Streptomyces peucetius. In order to increase doxurubicin productivity, a semi-industrial doxorubicin-producing Streptomyces strain named BR-Dox was cultured in a R2YE liquid medium containing CaCO, and then converted to a cell wall-free protoplast using lysozyme treatment method, followed by PEG-mediated cell wall regeneration. Among several protoplast-regenerated Streptomyces BR-Dox strains, two independent isolates named BR-Dox4 and BR-Dox6 were visually selected using thin layer chromatography (TLC) based on the pigment overproducing phenotype. Comparing with Streptomyces BR-Dox parental strain, two protoplast-regenerated strains, BR-Dox4 and BR-Dox6 exhibited 25.2％ and 12.2％ higher doxorubicin productivity analyzed by high pressure liquid chromatography (HPLC), respectively. This result suggests that a protoplast-regeneration of an antibiotics-producing Streptomyces strain should be a promising strain development approach for antibiotics overproduction in Streptomyces species.
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KEYWORD
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Protoplast regeneration, Doxorubicin, Streptomyces peucetius
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